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In addition of being tedious and lengthy, the process of batch digestion of proteinaceous species is also froth with the contamination of enzymes auto digests.
It is well understood that as the digestion proceeds in batch, the auto-digested enzymes no longer have a similar behavior as the intact enzymes.
Moreover, batch digestion is an anachronistic process that should no longer remain in the lexicon of today’s concept of real-time modus operandi.
To highlight the point, we have plotted the previously digested Trypsin on the immobilized Trypsin reactor, along with the digests of Cytochrome c from equine using similar conditions in both cases.

See Application Note 144:

Enzyme auto-digestion during batch mode processes. Assessment of the level of contamination comparing it to the digests of Cytochrome c from equine as an example.

How would a Trypsin solution behave and how would it affect the immobilized Trypsin on the column.
We have, in this application note, look at this issue.

See Application Note 142:

Automated Digestion of Trypsin with StyrosZyme® TPCK-Trypsin, Immobilized Enzyme on Polymeric Hard Gel Simulated-Monolith™ Enzyme Reactor with the Acquity UPLC I class Plus and Silica mapping.

If we were to add the Tryspsin solution in excess, would that digest the immobilized Trypsin?
This Application Note clarifies this question.

See Application Note 153:

Automated Digestion of Trypsin with StyrosZyme® TPCK-Trypsin, Immobilized Enzyme on Polymeric Hard Gel Simulated-Monolith™ Enzyme Reactor with the Acquity UPLC I class Plus and Final Silica mapping. Result of excess injection of Trypsin on the Trypsin column.

Heartcutting is commonly used to specifically remove components from a mixture without resorting to affinity columns or the like.  
It is however promoted as a somewhat involved process. 
We are offering a simple alternative here: 

See Application Note 147:

Heartcutting Made Simple Using Acquity UPLC I class Plus.

Alternatively using a different instrument with the same 6 port valve it is possible to show the process is universal.

See Application Note 148:

Heartcutting Made Simple Using Agilent 1290 Infinity.

Identifying small amounts of chemicals in large volumes has been a time consuming and resource wasting process that is cluttering the relevant industries for some time.
Solid phase extraction coupled with high performance polymerics on the same column is the proper alternative.

See Application Note 149:

Trap Concentrate and Map, Using Acquity UPLC I class Plus

Comparing it to a Silica C18 column:

See Application Note 150:

Trap, Concentrate and Map, Using Acquity UPLC I class Plus. STYROS® R Polymeric Compared with C18 Acquity UPLC® BEH.

And a follow up:

See Application Note 151:

Trap, Concentrate and Map, Using Acquity UPLC I class Plus. STYROS® R Polymeric Compared with C18 Acquity UPLC® BEH. Folow up Study of App. Note 150.

More follow up:

See Application Note 152:

Trap, Concentrate and Map, Using Acquity UPLC I class Plus. STYROS® R Polymeric Compared with C18 Acquity UPLC® BEH. Folow up Study of App. Note 150, 151.