StyrosZyme® TPCK-Trypsin
Technical Specifications
|
Support Matrix |
Hydrophilic coated cross linked polystyrene/divinyl benzene |
|
Loading |
6-8 mg per ml of bed volume |
|
Surface Functionality |
TPCK-Trypsin |
|
Shipping solvent |
0.02 % sodium azide in DI Water or 10% MeOH in DI Water. |
|
Packing density |
0.4 g/ml |
|
Shrinkage/swelling |
< 0.5% under different salt or solvent concentrations. |
General Considerations
At pH 7-8, Trypsin cleaves the peptide bonds of the protein at the carboxy terminus of arginine, lysine, and S-2-aminoethylcysteine residues.
At pH 11 the cleavage is more selective to lysyl residues in the presence of S-2-aminoethylcysteine.
Enzyme digestion varies with proteins as well as the operating conditions such as temperature, flow rate and pH.
By controlling these variables, it is possible to control the extent of digestion.
StyrosZyme® TPCK-Trypsin can be ordered in PEEK or Stainless Steel Columns.
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