STYROS® rA (recombinant Protein A)
STYROS® rA (Immobilized Recombinant Protein A)
Cost, lifespan and leaching were considered in the development of this new Affinity chromatography product with a Simulated Monolith™ matrix.
§ Product summary:
STYROS® rA are the first Simulated Monolith™ packed columns made of polymeric media intended for analytical and preparative affinity purification of antibodies.
The base monolithic matrix that is fully pervious is made of highly cross-linked poly (styrene-divinylbenzene).
Typical linear flow rates are 1,800 cm/hr and above, compared to 140 cm/hr with soft gel.
Unlike Monolith, there are no limitations with Simulated Monolith™ columns. They are available in most sizes and configurations.
Higher practical speed and efficient use of the column is another advantage of such products.
STYROS® rA media are available in different sizes.
§ Key Features:
§ Feature 1:
Dynamic binding capacity @ 3,600 cm/hr: 35-40 mg/ml.
§ Feature 2:
Although we have used pressures of up to 10,000 psi without irreversibly altering the structure of the bed, we recommend that the pressure be kept under 2,000 psi during the loading and unloading of the columns.
§ Feature 3:
No measurable shrinking and/or swelling due to highly cross-linked nature of the beads.
§ Specifications:
Support Matrix | Cross-linked Poly(styrene-divinylbenzene) |
Surface Functionalities | Tethered recombinant protein A |
Binding Capacity | 35-40 mg/ml Human IgG @ pH=7.5 |
Shipping Solvent | 10 % MeOH in DI Water |
Packing Density | 0.4 g/ml |
Shrinkage/Swelling | < 0.2 % under different salt or solvent concentrations |
Maximum Pressure Tolerance | 140 bar (2,000 psi, 14 MPa) |
STYROS® rA are provided in PEEK and Stainless Steel columns of different sizes.
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